Cited 0 times in

66 22

Detection of Mycobacterium kansasii Using DNA-DNA Detection of Mycobacterium kansasii Using DNA-DNA Hybridization with rpoB Probe

Authors
 Tae-Dong Kweon ; Sun-Joon Bai ; Seong-Karp Hong ; Chang-Shik Choi 
Citation
 Journal of information and communication convergence Engineering, Vol.10(2) : 210~214, 2012 
Journal Title
 Journal of information and communication convergence Engineering 
ISSN
 2234-8255 
Issue Date
2012
Abstract
A microtiter well plate DNA hybridization method using Mycobacterium kansasii-specific rpoB DNA probe (kanp) were evaluated for the detection of M. kansasii from culture isolates. Among the 201 isolates tested by this method, 27 strains show positive results for M. kansasii, but the other 174 isolates were negative results for M. kansasii. This result was consistent with partial rpoB sequence analysis of M. kansasii and the result of biochemical tests. The negative strains by this DNA-DNA hybridization method were identified as Mycobacterium tuberculosis (159 strains), Mycobacterium avim (5 strains), Mycobacterium intracellulare (8 strains), and Mycobacterium flavescens (2 strain) by rpoB DNA sequence analysis. Due to high sensitivity and specificity of this test result, we suggest that DNA-DNA hybridization method using rpoB DNA probes of M. kansasii could be used for the rapid and convenient detection of M. kansasii.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/90856
DOI
10.6109/jicce.2012.10.2.205
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Anesthesiology and Pain Medicine
Yonsei Authors
사서에게 알리기
  feedback
Files in This Item:
T201203182.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse